Our Proteomics Services
At RxCelerate, we take the time to thoroughly understand the unique context and nuances of each client’s challenge before designing a customised solution.
Proteomics is a powerful technique that has great power to unlock multiple phases of the drug development process. However, it is also a complex procedure that requires considerable expertise to master. Through our proteomics solutions, we work with you to apply our proteomics expertise to meet your objectives.
In-house Technologies
We do not limit ourselves to protocols and analytical methods used by others. We have a range of proprietary and patented methods developed in-house for improved analytical performance and unique outputs. Together these comprise the ProQuant® proteomics platform.
Comprehensive Proteomics Insights
Targeted proteomics from major platform providers wholly or predominantly focus on protein abundance, overlooking the dynamic nature of the proteome which is shaped not just by DNA-encoded proteins, but by mRNA and post-translational modifications (both regulated and incidental) that define true biological complexity.
With ProQuant® we can optimise for depth of protein coverage, detecting and providing measurements of >6000 proteins in tissues. Alternatively, we can focus on investigating proteoform-level features, potentially revealing thousands of endogenous PTMs.
Applications of Proteomics
Using the ProQuant® platform, we deliver high-performance, tailored proteomics for drug development, with a wide range of applications.
Biomarker Identification
We measure total proteins, specific PTMs, subtle patterns of PTMs, and endogenous protein cleavages to maximise the chance of identifying biomarkers missed by conventional abundance-only approaches.
Chemoproteomics
The original patent-protected ProQuant® method was designed to detect and quantify protein modifications, making it ideal for identifying and characterising covalent drug interactions at specific amino acid sites.
Characterising ProTACs
Using ProQuant®, we can monitor both on- and off-target protein degradation by PROTACs, helping characterise their selectivity and mechanism of action.
Quantifying protease activity
ProQuant® can identify and quantify peptides generated from endogenous proteases, enabling us to estimate protease activity in vitro and in vivo – something that’s challenging to achieve with other technologies.
Biologics CMC
We offer exceptional PTM quantification, including measurement of isoaspartic formation in complex mixtures such as plasma or tissues, a capability we have found no published precedent for.
Biologics development
The ProQuant® platform enables tracking of PTMs on biological drugs post-dosing, revealing effects on PK, activity, and anti-drug antibody formation.
Characterisation of labels
Many techniques depend on labelling proteins covalently, Using the ProQuant® platform we can rapidly visualise the locations of labels and site occupancy, with exceptional precision and accuracy.
And that's not all...
Access to high-performance proteomics platforms like ProQuant® makes it possible to find insights you may not have known were possible. Whether it’s CMC of a complex biological agent, PK for a protein therapeutic that is undergoing modification in vivo, or a purely academic attempt to understand the conformational dynamics associated with activity of an intriguing protein, we can unblock your discovery or development pipeline. We will leverage the power of the ProQuant® platform to answer the questions that matter most to you.